3D-Protein Chips Based on Microstructured Surface-Attached Polymer Networks. Oberflächenchemie und -physik von Mikrosystemen Bd. 3 # Pb., Großformat (24x17), 132 S., 53 Abb., 8 Tab.
This volume describes a new technique to produce protein chips on polymer substrates by immobilization of proteins in surface-attached polymer networks. Nanoliter droplets of polymer and protein are dispensed and attached to the surface by photocrosslinking with UV-light and thus the proteins are simultaneously immobilized. The water swellable polymer network acts as a three-dimensional flexible scaffold for the proteins that makes the immobilized antibodies accessible for the reaction with antigen from solution.
The microstructured protein chips show high signal to noise and signal to background ratios in immunoassays with fluorescence read-out. The measured fluorescence intensity can be adapted by changing the composition and the height of the microstructures. This allows to measure a broad range of antigen concentrations on these chips using an in-situ fluorescence measurement setup where surface-bound fluorophores are excited by the evanescent field of light guided inside the transparent chip.