Buchhop, Jutta:
ISBN 9783899599299

Characterization of phylogenetically diverse CLRV-isolates by RFLP and research into identification of two isometric viruses. Berliner ökophysiologische und phytomedizinische Schriften Bd. 5 # Pb., 124 S., 26 Abb., 35 Tab.

SCHLAGWORTE:
cherry Leafroll virus
tree virus
herba ceous propagation host
restriction fragment length polymorphism
phylogenetic groups
Brome mosaic virus

Cherry leaf roll virus (CLRV) is a virus with a wide geographic distribution and a wide host range. It has been shown in the past that isolates differ in host range as well as serotype. Furthermore, phylogenetic groups can be distinguished that depend largely on the original host plant. In this study 3 different virus isolates that were originally derived from rhubarb, cherry and black elderberry were assessed for differences in symptom development on different herbaceous biotest hosts. To enable differentiation of CLRV isolates belonging to different phylogenetic groups an RFLP assay was established using 43 plasmid constructs containing a 420 bp fragment from the 3'NCR of different CLRV isolates. In an immunocapture-reverse transcription-PCR-restriction fragment length polymorphism-assay the method was applied to distinguish unknown CLRV isolates derived from walnut, black elderberry and birch.
Moreover, identification of unknown viruses that contaminated two CLRV isolates was aspired by arbitrary primed PCR and PCR using specific primers and let to identification of one of the contaminating viruses as brome mosaic virus.





Cherry leaf roll virus (CLRV) is a virus with a wide geographic distribution and a wide host range. It has been shown in the past that isolates differ in host range as well as serotype. Furthermore, phylogenetic groups can be distinguished that depend largely on the original host plant. In this study 3 different virus isolates that were originally derived from rhubarb, cherry and black elderberry were assessed for differences in symptom development on different herbaceous biotest hosts. To enable differentiation of CLRV isolates belonging to different phylogenetic groups an RFLP assay was established using 43 plasmid constructs containing a 420 bp fragment from the 3'NCR of different CLRV isolates. In an immunocapture-reverse transcription-PCR-restriction fragment length polymorphism-assay the method was applied to distinguish unknown CLRV isolates derived from walnut, black elderberry and birch.
Moreover, identification of unknown viruses that contaminated two CLRV isolates was aspired by arbitrary primed PCR and PCR using specific primers and let to identification of one of the contaminating viruses as brome mosaic virus.

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